Company Collaborates with Ghent University to Incorporate LNCipedia Content
SANTA CLARA, Calif., June 8, 2015
Agilent Technologies Inc. (NYSE: A) today announced updates to its SurePrint gene expression microarrays for messenger RNA profiling applications for human, mouse and rat models. The updates improve coding and noncoding content, which will provide up-to-date tools for researchers to investigate expression patterns on a highly accessible platform.
Agilent developed the updates to its flagship SurePrint G3 Human v3 gene expression microarray in collaboration with Ghent University for complete coverage of the LNCipedia 2.1 database to assay high-confidence, long noncoding RNA (lncRNA) transcripts.
LncRNAs (noncoding RNA longer than 200 nucleotides) modify gene regulation through direct interactions with DNA, RNA and proteins to enable target-specific or system-wide modulation. The broad but critical role of lncRNAs can be seen in their association with cancer, cardiovascular and neurodegenerative diseases.
Agilent’s redesigned gene expression microarrays are high-quality signature capture tools for efficient analysis of genes or pathways of interest with broad applications that range from aiding in disease stratification to elucidating drug mechanisms.
“We worked closely with Agilent to design a best-in-class profiling assay for mRNA and lncRNA expression,” said professor Jo Vandesompele of Ghent University. “Measuring both types of RNA in a single assay provides insight into the biological association between mRNA and lncRNA from relative expression levels. The key is having a good balance of coding and long noncoding features, and LNCipedia 2.1 is an optimal source to pair with Agilent’s gene expression content. The final microarray design is optimized and provides a lot of valuable information quickly.”
The updated microarrays are fabricated using SurePrint technology, which enables precision oligonucleotide synthesis. SurePrint microarrays have industry-leading sensitivity with greater than 5 logs of dynamic range, high reproducibility with an interarray median coefficient of variation of 5 percent, and excellent quantitative concordance with the External RNA Controls Consortium’s spike-in controls at R2>0.95.
“Our SurePrint gene expression microarrays encompass not only the rigor of expert content, such as LNCipedia’s lncRNAs, but also the flexibility of customization for expert users,” said Dr. Alessandro Borsatti, senior director of genomics at Agilent. “Through the high performance and quantitative concordance of our gene expression microarrays, along with our RNA-Seq and target-enrichment products, we enable researchers to smoothly transition between microarray screening applications and deeper next-generation sequencing discovery.”
SurePrint gene expression microarrays are part of the SurePrint product family, which includes microRNA and comparative genomic hybridization microarrays. Agilent’s genomic workflow includes the 2100 Bioanalyzer and 2200 Tapestation for quality control, SureScan scanner for data acquisition, GeneSpring software for data analysis, and the AriaMX system for real-time polymerase chain reactions.